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Synthesis, isolation and immunochemical characterization of N‐(carboxymethyl‐ 3 H)‐ D ‐lysergamide
Author(s) -
Winkelhake Jeffrey L.,
Voss Edward W.
Publication year - 1974
Publication title -
journal of labelled compounds
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0022-2135
DOI - 10.1002/jlcr.2590100315
Subject(s) - lysergic acid , carbodiimide , moiety , chemistry , stereochemistry , amide , covalent bond , lysergic acid diethylamide , glycine , biochemistry , amino acid , organic chemistry , receptor , serotonin
Covalent amide linkage of glycine‐2‐ 3 H to the non‐hallucinogenic derivative, d‐lysergic acid (LSA), by carbodiimide activation results in a lysergic acid analogue which has potential use in biochemical studies of the mode of action, metabolism and immunology of d ‐lysergic acid diethylamide (LSD). Optimal conditions for the reaction and isolation of the product, N‐(carboxymethyl‐ 3 H)‐ d ‐lysergamide, were determined. The product was analyzed by radioimmune assay and by equilibrium dialysis with highly specific antibody to the lysergyl moiety.