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Preparation Microbiologique Du 2‐CETO‐3‐Desoxy‐D‐Gluconate 1‐ 14 C ou U‐ 14 C
Author(s) -
Pouyssegur J.
Publication year - 1973
Publication title -
journal of labelled compounds
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0022-2135
DOI - 10.1002/jlcr.2590090102
Subject(s) - glucuronate , chemistry , yield (engineering) , specific activity , radiochemistry , chromatography , biochemistry , enzyme , materials science , metallurgy
A E. Coli K 12 mutant having lost 2‐keto‐3‐desoxy‐gluconate kinase activity is unable to utilize glucuronate as a unique carbon source. However, this strain is able to partially convert glucuronate into KDG in resting cells conditions reported here. A method is described making it possible to produce 1‐ 14 C‐KDG or U‐ 14 C‐KDG when the kinase cells metabolise 6‐ 14 C or U‐ 14 C‐D‐glucuronate. The radioactive KDG accumulated in the incubation medium has been purified thanks to a biological specific method followed by chromatography on anionic resin. The yield of the purified KDG from the hexuronate is about 20%. According to chromatographic criteria and specific conversion by KDG‐kinase, the product obtained has been authenticated as true KDG.