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Arginine‐rich polypeptides in saline extract of chlorella pyrenoidosa‐ 14 C
Author(s) -
Nejedly Zdenek,
Hybs Karel
Publication year - 1972
Publication title -
journal of labelled compounds
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0022-2135
DOI - 10.1002/jlcr.2590080204
Subject(s) - arginine , chlorella pyrenoidosa , chlorella , biochemistry , protamine , chemistry , glutamic acid , sodium , protamine sulfate , aspartic acid , lysine , nucleic acid , amino acid , serine , chromatography , biology , organic chemistry , heparin , algae , enzyme , botany
Abstract The radiochromatographic analysis of the hot 10% sodium chloride extract of the alga Chlorella pyrenoidosa‐ 14 C indicated the presence of compounds rich in L‐arginine, besides of nucleic acids and glucose‐type polysaccharide estimated previously. L‐Arginine is present in a bound form, most likely as a protamine‐type polypeptide or a histone‐type protein containing L‐arginine as a dominant component, besides of smaller amounts of L‐glutamic acid, L‐aspartic acid and L‐serine, A simple procedure of the isolation of L‐arginine‐ 14 C/U/ from the saline extract of radioactive Chlorella cells, on a preparative scale, is described.