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The preparation of tritium‐labelled ryanodine
Author(s) -
Fairhurst Alan S.
Publication year - 1971
Publication title -
journal of labelled compounds
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0022-2135
DOI - 10.1002/jlcr.2590070205
Subject(s) - tritium , chemistry , ryanodine receptor , yield (engineering) , thin layer chromatography , radiochemistry , alkaloid , bromine , specific activity , thin layer , chromatography , aqueous medium , aqueous solution , nuclear chemistry , organic chemistry , calcium , layer (electronics) , materials science , enzyme , physics , nuclear physics , metallurgy
A method for preparing tritium‐labelled ryanodine is described. The alkaloid was first brominated, and the bromoryanodines were separated from the reaction mixture by thin layer chromatography; NMR analysis at this stage showed substitution of pyrrole protons by bromine. Bromoryanodine was first hydrogenated, and the product was purified and characterized as ryanodine, to establish the feasibility of the method. Tritium was then reacted with bromoryanodine to yield labelled ryanodine with a specific activity of 2.85 Ci/mmole. No detectable isotope exchange occurred when the labelled alkaloid was incubated in aqueous media at pH 7.0 and 30° C.