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The synthesis of some pyrimidine derivatives labelled with 14 C
Author(s) -
Jezdić V.,
Razumenić N.,
Skakun M.,
Albahari S.,
OdavićJosić J.
Publication year - 1970
Publication title -
journal of labelled compounds
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0022-2135
DOI - 10.1002/jlcr.2590060112
Subject(s) - orotic acid , uracil , pyrimidine , thymine , thiouracil , chemistry , nitrogen , homogeneous , carbon 14 , radiochemistry , organic chemistry , stereochemistry , biochemistry , dna , physics , thyroid , thermodynamics , medicine , quantum mechanics
The procedures for synthesis of the following pyrimidine derivatives have been described (yields are given in brackets) : 2‐thiouracil‐2‐ 14 C (94 %), uracil‐2‐ 14 C (91 %), 2‐thiothymine‐2‐ 14 C (92 %), thymine‐2‐ 14 C (90 %), 5‐bromouracil‐2‐ 14 C (95 %) and orotic acid‐6‐ 14 C (68 %). All these substances are chemically and radiochemically homogeneous and pure. The procedures develop according to the known reaction schemes with some modifications offering better yields. A new apparatus has been constructed for the synthesis of orotic acid‐6‐ 14 C enabling continuous work in the dry nitrogen atmosphere. Specific activities are 28–30 mCi/mmole. The activities used were 60 mCi, and for the orotic acid‐6‐ 14 C 30–180 mCi.