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Validity of tritium tracers. Stability of tritium atoms in purines, pyrimidines, nucleosides and nucleotides
Author(s) -
Anthony Evans E.,
Sheppard Hilary C.,
Turner John C.
Publication year - 1970
Publication title -
journal of labelled compounds
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0022-2135
DOI - 10.1002/jlcr.2590060111
Subject(s) - tritium , chemistry , nucleotide , nucleic acid , purine metabolism , uridine , tritiated water , radiolysis , radiochemistry , decomposition , organic chemistry , biochemistry , rna , aqueous solution , physics , nuclear physics , gene , enzyme
The effect of pH and temperature on the stability of tritium atoms at various positions in purines, pyrimidines, nucleosides and nucleotides is discussed. The importance of recognizing several types of instability is stressed. The formation of a hydrate on storing solutions of tritiated uridine and other results of the self‐radiolysis of tritiated precursors of the nucleic acids are described. Alcohol solutions and low temperatures are recommended for minimising self‐decomposition.