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Preparation of riboflavin (UL)‐ 14 C
Author(s) -
Lowry L. K.,
Herman Y. F.,
Sauberlich H. E.
Publication year - 1969
Publication title -
journal of labelled compounds
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0022-2135
DOI - 10.1002/jlcr.2590050411
Subject(s) - riboflavin , chemistry , vitamin , biochemistry , degradation (telecommunications) , chromatography , telecommunications , computer science
Uniformly labeled riboflavin‐ 14 C was synthesized by the organism, Ashbya gossypii. Riboflavin with a specific activity of 79.0 mCi/mmole was obtained after extensive purification. Degradation of riboflavin with light and NaOH indicated that the 14 C label was present in both the isoalloxazine ring and in the side chain. Radioautographs of the chromatographed vitamin showed that a single radioactive band was found that coincided with authentic riboflavin.