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Liquid tritioammonia as a tritiating agent
Author(s) -
Bloss K.
Publication year - 1969
Publication title -
journal of labelled compounds
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0022-2135
DOI - 10.1002/jlcr.2590050410
Subject(s) - liquid scintillation counting , piperidine , chemistry , toluene , asparagine , glutamine , chromatography , nuclear chemistry , organic chemistry , amino acid , biochemistry
Tritioammonia, 10 Ci/g, tritiated piperidine, 5‐hydroxytryptophan, glutamine, and asparagine by refluxing at −35 °C. Within 1.5 to 6 hours the specific activities reached 0.02, 0.775, 1.24 and 2.48 mCi/mmole, purified. Cyclooctatetraene was not exchangelabeled under these conditions. For liquid‐scintillation counting of tritioammonia, ortho‐bromobenzoic acid in toluene‐dioxan served well. Autoradiolysis was no problem.