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Synthesis of glucose‐3 and ‐4‐ 14 C of high specific activity and radiochemical purity
Author(s) -
Sturani E.
Publication year - 1969
Publication title -
journal of labelled compounds
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0022-2135
DOI - 10.1002/jlcr.2590050108
Subject(s) - aldolase a , glyceraldehyde , chemistry , biochemistry , ribulose , enzyme , pyruvate carboxylase , organic chemistry , dehydrogenase
A method for the enzymatic synthesis of hexoses‐3‐ 14 C and hexoses‐4‐ 14 C is presented. The procedure is based on the synthesis of 3‐phosphoglyceric acid‐1‐ 14 C (3PGA) by the action of the ribulose diphosphate carboxylase in the presence of ribulose‐1,5 diphosphate and 14 CO 2 , followed by the reversal of the glycolytic reactions leading from glucose to 3PGA. For the synthesis of hexoses‐3‐ 14 C, dihydroxyacetonephosphate‐1‐ 14 C and cold glyceraldehyde‐3‐P are condensed by the aldolase reaction, while for the synthesis of hexoses‐4‐ 14 C cold dihydroxyacetonephosphate is condensed with glyceraldehyde‐3‐P‐1‐ 14 C. The problems and the results of such syntheses are discussed.