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Synthèse du linolènate et de l'arachidonate de cholestéryle doublement marqués
Author(s) -
Bardou L.,
De Paulet A. Crastes
Publication year - 1965
Publication title -
journal of labelled compounds
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0022-2135
DOI - 10.1002/jlcr.2590010108
Subject(s) - linolenate , chemistry , transesterification , cholesterol , fatty acid , arachidonic acid , cholesteryl ester , biochemistry , enzyme , catalysis , lipoprotein
The transesterification method used by M AHADEVAN and L UNDBERG for cholesterol esters synthesis, following the scheme cholesterol acetate + fatty acid methyl ester → cholesterol ester + methyl acetate, has been adapted to the microscale (1 to 10 μM) imposed by the use of labelled molecules of high specific activity. This method is particularly suitable for the synthesis of polythylenic fatty acid cholesterol esters: it has unable us to obtain the following esters: 4 14 C and 7α 3 H cholesteryl linolenate and arachidonate, cholesteryl linolenate U 14 C and arachidonate (5‐6, 8‐9, 11‐12, 14‐15) 3 H; the mixture of these two types of esters of high specific activity give rise to series of doubly labelled esters, at the user's convenience.