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Cellular uptake and processing of directly and indirectly 125 I‐iodinated and 76 BR‐brominated monoclonal antibody A33
Author(s) -
Höglund J.,
Tolmachev V.,
Orlova A.,
Lundqvist H.,
Sundin A.
Publication year - 2001
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.25804401251
Subject(s) - chemistry , monoclonal antibody , intracellular , antibody , stereochemistry , biochemistry , immunology , biology
Abstract In this study the binding pattern and intracellular retention of the monoclonal antibody (MAb) A33 were investigated. The antibody was labelled with 125 I or 76 Br using either the conventional direct Chloramine‐T method or indirectly by conjugation of a radiohalogenated precursor resulting in 125 I‐A33, [ 125 I] para ‐iodobenzoate‐A33, 76 Br‐A33 or [ 76 Br]‐ para ‐bromo‐benzoate‐A33, respectively. All labelled molecules bound specifically to SW1222 cells although the cellular retention of the different labels varied, with slower excretion of the radiolabel when indirect labeling of the MAb was applied. In conclusion, indirect labeling of MAbs leeds to a higher uptake and a prolonged cellular retention.