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Rutin labeled with technetium‐99M and its biodistribution in wistar rats
Author(s) -
Bernardo L. C.,
Oliveira M. B. N.,
da Silva C. R.,
Moura R. S.,
BernardoFilho M.
Publication year - 2001
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.25804401228
Subject(s) - rutin , biodistribution , chemistry , technetium 99m , free radical scavenger , technetium , antioxidant , flavonoid , chromatography , pertechnetate , pharmacology , radiochemistry , biochemistry , nuclear chemistry , nuclear medicine , medicine , scintigraphy , in vitro
The use of natural products as medicines is growing along the years in the world. The rutin, a compound isolated from Ruta graveolens , is a flavonoid which has been suggested to have the capacity to protect the capillary endothelium and to reduce the triacylglycerol levels. Besides, it seems to present antioxidant characteristics and to be a great free radical scavenger. The use of technetium‐99m (99mTc) can be justified for its physical and chemistry properties. The aim of this study is to label rutin with 99mTc and to study its biodistribution in Wistar rats. Firstly, rutin was dissolved in ethyl alcohol, and later incubated with stannous chloride. After that, 99mTc, as sodium pertechnetate, was added. The labeling efficiency was determined through the instant thin layer chromatography (ITLC‐SG). The biodistribution study of the rutin labeled with 99mTc was performed with Wistar rats and the highest uptake was found in kidneys (0.535±0.09 % ID/g). It is possible to conclude that the rutin was successfully labeled with 99mTc.