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Chemical modifications of 99m Tc tricarbonyl labeled single chain antibody fragments for improved in vivo behavior in tumor targeting
Author(s) -
Stichelberger A.,
Jager H.,
Waibel R.,
NovakHofer I.,
Chester K.,
Schubiger P. A.
Publication year - 2001
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.25804401199
Subject(s) - chemistry , in vivo , succinic anhydride , antibody , kinetics , penetration (warfare) , biophysics , radiochemistry , organic chemistry , immunology , physics , microbiology and biotechnology , quantum mechanics , operations research , engineering , biology
One of the major limitations of using intact immunoglobulins for targeting tumors is the poor penetration into tissues. Although small single chain Fv (scFv) antibody fragments have been used because of their improved kinetics, some exhibit high renal accumulation. In this study we show a significant reduction of renal accumulation of His‐tagged scFv's directly labelled with 99m Tc‐tricarbonyl by use of succinic anhydride. Further decrease of renal uptake will make this approach hopefully suitable for therapeutic application, using 188/186 Re(I)‐tricarbonyl.