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An improved method for hyaluronic acid radioiodination
Author(s) -
Orlando P.,
Binaglia L.,
De Feo A.,
Orlando M.,
Trenta R.,
Trevisi R.
Publication year - 1995
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580360906
Subject(s) - hyaluronic acid , chemistry , sodium cyanoborohydride , yield (engineering) , specific activity , chromatography , combinatorial chemistry , biochemistry , organic chemistry , enzyme , genetics , materials science , metallurgy , biology
A simple procedure is described for preparing 125 I‐labelling hyaluronan of high molecular weight. The reducing terminal group of hyaluronic acid was derivatized with tyramine through the formation of a Schiff base which was subsequently reduced with sodium cyanoborohydride. By radioiodination of the aromatic ring, 125 I‐labelled hyaluronic acid was obtained in high yield (40%) and high specific activity, 555 GBq/mmol (15 Ci/mmol).