Synthesis of selectively  13 C‐labelled benzoic acid for nuclear magnetic resonance spectroscopic measurement of glycine conjugation activity | Zendy

Akira Kazuki | Zendy; Hasegawa Hiroshi | Zendy; Baba Shigeo | Zendy

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Synthesis of selectively 13 C‐labelled benzoic acid for nuclear magnetic resonance spectroscopic measurement of glycine conjugation activity

Author(s) -

Akira Kazuki,

Hasegawa Hiroshi,

Baba Shigeo

Publication year - 1995

Publication title -

journal of labelled compounds and radiopharmaceuticals

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.432

H-Index - 47

eISSN - 1099-1344

pISSN - 0362-4803

DOI - 10.1002/jlcr.2580360905

Subject(s) - chemistry , benzoic acid , hippuric acid , carbon 13 , glycine , protonation , nuclear magnetic resonance spectroscopy , yield (engineering) , nuclear chemistry , acetone , nuclear magnetic resonance , radiochemistry , stereochemistry , organic chemistry , amino acid , urine , biochemistry , ion , physics , materials science , quantum mechanics , metallurgy

The synthesis of [4‐ 13 C]benzoic acid (BA) labelled in a single protonated carbon, for use as a probe to measure glycine conjugation activity by nuclear magnetic resonance (NMR) spectroscopy, has been reported. The labelled compound was prepared by a seven‐step synthetic scheme on a relatively small scale using [2‐ 13 C]acetone as the source of label in overall yield of 16 %. The usefulness of [4‐ 13 C]BA was demonstrated by the NMR spectroscopic monitoring of urinary excretion of [4‐ 13 C]hippuric acid in the rat administered with the labelled BA.

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