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Synthesis and purification of 13 C labelled xanthine derivatives
Author(s) -
Boukraa M. S.,
Deruaz D.,
Bannier A.,
Desage M.,
Brazier J. L.
Publication year - 1995
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580360809
Subject(s) - xanthine , chemistry , theobromine , theophylline , ibmx , high performance liquid chromatography , nuclear chemistry , chromatography , organic chemistry , biochemistry , enzyme , medicine , receptor , forskolin , endocrinology
3‐[Methyl‐ 13 C]xanthine, 7‐[Methyl‐ 13 C]xanthe, 1,3‐[Dimethyl‐ 13 C 2 ]xanthine (theophylline‐1,3‐[ 13 CH 3 ] 2 ), 1,7‐[Dimethyl‐ 13 C 2 ]xanthine (paraxanthine‐1,7‐[ 13 CH 3 ] 2 ), and 3,7‐[Dimethyl‐ 13 C 2 ]xanthine (theobromine‐3,7‐[ 13 CH 3 ] 2 ) were synthesized by nucleophilic substitution reaction (SN 2 ) from xanthine (X) and iodomethane‐[ 13 C]. The 3‐isobutylparaxanthine‐7‐[ 13 CH 3 ] was prepared from 3‐isobutyl‐1‐methylxanthine (IBMX). The compounds were purified by reverse phase semipreparative liquid chromatography and their chemical structure and purity verified by GC‐MS.