Premium
Application of solid state catalytic hydrogen isotope exchange to the tritium labeling of lysozyme
Author(s) -
Filikov Anton V.,
Jones John R.,
Myasoedov Nikolai F.,
Ward E. Sally
Publication year - 1995
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580360210
Subject(s) - chemistry , tritium , lysozyme , catalysis , specific activity , peptide , radiochemistry , tritium illumination , isotope , hydrogen isotope , hydrogen , solid state , tritiated water , enzyme , chromatography , organic chemistry , biochemistry , physics , quantum mechanics , nuclear physics
Solid state catalytic hydrogen isotope exchange has been employed to label hen egg lysozyme with tritium. Optimization of reaction conditions so that amino acids and peptide bonds remained intact led to a tritiated product with 97% of the original enzymatic activity and 94% radiochemical purity. The specific activity when using a T 2 :H 2 mixture of 1:1000, was 16 mCi·mmol −1 . It is suggested that the currently adopted approach may have wide applications for other proteins able to tolerate lyophilization conditions without loss of activity.