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A practical method for the synthesis and purification of 14 C labeled oligonucleotides
Author(s) -
Sasmor Henri M.,
Dellinger Douglas J.,
Zenk Paul C.,
Lee Larry P.
Publication year - 1995
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580360103
Subject(s) - phosphoramidite , oligonucleotide , chemistry , nucleoside , thymidine , oligonucleotide synthesis , combinatorial chemistry , solid phase synthesis , dna synthesis , chromatography , biochemistry , dna , peptide
A practical method for the solid‐phase synthesis and purification of 14 C labeled oligonucleotides is described. A streamlined procedure for the synthesis of 5′‐O‐dimethoxytrityl‐2‐( 14 C)‐thymidine 3′‐(2‐cyanoethyl‐N,N‐diisopropyl) phosphoramidite from commercially available 2‐( 14 C) labeled thymidine nucleoside is detailed. Methodology for the manual synthesis, isolation, and purification of a high specific activity oligonucleotide phosphorothioate 20‐mer (ISIS 2105) are elaborated.