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Isolation and preparation of 3 H‐tetrandrine
Author(s) -
Huang Xun Yang,
Wang Yin,
De Lu Zang
Publication year - 1994
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580341002
Subject(s) - tetrandrine , chemistry , hydroxide , citric acid , chromatography , sodium hydroxide , isolation (microbiology) , organic chemistry , medicine , pathology , microbiology and biotechnology , biology
Tetrandrine is a bisbenzytehahydroisoquinoline alkaloid. Its structure is very complex and the preparation of labeled derivatives is difficult. We have prepared 3 H‐tetrandrine using the tritium gas exposure method. 1 This method produces many fragments which make isolation and purification of small quantities difficult. We investigated these fragments of unlabeled d‐tetrandrine and found almost all contain one to several hydroxide groups. Using this information, we designed a two‐step paper chromatography method for isolation and purification of 3 H‐Tetrandrine. This method involves the application of a pH 4.7 Na 2 HPO 4 ‐citric acid buffer to the paper to block the migration of hydroxide group fragments of tetrandrine. 3 H‐tetrandrine was successfully with a radiochemical purity of 90%.