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Radiolabeling kit/generator for 5‐radiohalogenated uridines
Author(s) -
BaranowskaKortylewicz Janina,
Helseth Lynn D.,
Lai Jing,
Schneiderman Martin H.,
Schneiderman G. Sue,
Dalrymple Glenn V.
Publication year - 1994
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580340604
Subject(s) - chemistry , deoxyuridine , bromine , chromatography , isotope , radiochemistry , generator (circuit theory) , halogenation , organic chemistry , biochemistry , dna , physics , quantum mechanics , power (physics)
A rapid, simple and inexpensive synthesis of 5‐radiohalogenated‐2′‐deoxyuridine from 5‐trimethylstannyl‐2′‐deoxyuridine is described. The total reaction and purification time including thin layer chromatography (tlc) for quality control is less than 30 min. This method produces excellent yields (>95%) of 123 I‐, 125 I‐, 131 I‐UdR. The radiochemical purity of all tested preparations (>20) was determined to be greater than 99%. This new method is the basis of a radiolabeling kit/generator for preparation of radiohalogenated nucleosides. 2′‐Deoxyuridine (UdR) halogenated with a stable isotope of bromine was also synthesized indicating that the method can be applied to the preparation of 5‐radiobromo‐2′‐deoxyuridine (BUdR).