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Synthesis of [4,5‐ 3 H‐Leu 4 ]Salmon Calcitonin
Author(s) -
Birtle T.,
White A. J.,
Woodhouse D. P.
Publication year - 1994
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580340412
Subject(s) - chemistry , tritium , residue (chemistry) , amide , leucine , peptide synthesis , peptide , solid phase synthesis , polyamide , specific activity , stereochemistry , radiochemistry , amino acid , organic chemistry , biochemistry , enzyme , physics , nuclear physics
The synthesis of salmon calcitonin, a C‐terminal amide dotriacontapeptide, labelled with tritium in the leucine residue at position 4 to a specific activity of 100Ci mmole −1 is described. The peptide was assembled using the Fmoc‐Polyamide solid‐phase synthesis strategy incorporating tritium labelled leucine, in the form of Fmoc‐4,5‐ 3 H‐leucinepentafluorophenylester, at the appropriate stage. The Fmoc‐4,5‐ 3 H‐leucinepentafluorophenylester was prepared via a multistage synthesis from 4,5‐dehydroleucine.