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Enzymatic synthesis of tritium — labelled prostaglandin D 2 and its conversion to other prostaglandins
Author(s) -
Shram Stanislav I.,
Lazurkina Tatyana Yu.,
Shevchenko Valerii P.,
Nagaev Igor Yu.,
Myasoedov Nikolai F.
Publication year - 1994
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580340407
Subject(s) - chemistry , tritium , enzyme , radiochemistry , prostaglandin , chromatography , biochemistry , nuclear physics , physics
The one‐stage enzymatic synthesis of tritium‐labelled prostaglandin D 2 from labelled arachidonic acid was performed by using the enzyme system PGH‐synthetase/PGH‐PGD‐isomerase. By enzymatic and chemical transformation of [ 3 H]PGD 2 the following compounds were obtained: 15‐keto‐13,14‐dihydro‐[ 3 PGD 2 , 9α,11β‐[ 3 H]PGD 2 , 9‐deoxy‐Δ 9 ‐[ 3 H]‐PGD 2 ([ 3 H]PGJ 2 ) and Δ 12 ‐13,14‐dihydro‐[ 3 H]PGJ 2 . It was found that L‐selectride is a more effective reducing agent than sodium borohydride in the synthesis of 9 α,11β‐[ 3 H]PGF 2 .