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Enzymatic and chemical labelling of prostaglandin E 1 and 13,14‐dihydro‐15‐keto‐prostaglandin E 1
Author(s) -
Tsikas Dimitrios,
Bracht Stefan,
Stichtenoth Dirk,
Frölich Jürgen C.
Publication year - 1993
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580331208
Subject(s) - chemistry , prostaglandin e , enzyme , labelling , endogeny , prostaglandin , chromatography , gas chromatography , extraction (chemistry) , mass spectrometry , gas chromatography–mass spectrometry , biochemistry
Chemical and enzymatic tools were employed to synthesize [1,1‐ 18 O 2 ]‐prostaglandin (PG) E1, [1,1‐ 18 O 2 ]‐13,14‐dihydro‐15‐keto‐PGE 1 and [5,6‐ 3 H 2 ]‐13,14‐dihydro‐15‐keto‐PGE 1 starting from the corresponding unlabelled compounds adn [5,6‐ 3 H 2 ]‐PGE 1 , respectively. Reaction products were purified by high‐performance liquid chromatography and their isotopic purity was determined by gas chromatographymass spectrometry (GC‐MS). The utility of [1,1‐ 18 O 2 ]‐PGE 1 and [1,1‐ 18 O 2 ]‐13,14‐dihydro‐15‐keto‐PGE 1 as internal standards for GC‐MS analysis of the corresponding endogenous compounds in biological fluids is demonstrated. [5,6‐ 3 H 2 ]‐13,14‐dihydro‐15‐keto‐PGE 1 was found to be useful in developing extraction and purification procedures for the quantitation of endogenous 13,14‐dihydro‐15‐keto‐PGE 1 in human plasma by GC‐tandem MS.