Premium
Low cost production of perdeuterated biomass using methylotrophic yeasts
Author(s) -
Haon Stéphane,
Augé Sophie,
Tropis Marielle,
Milon Alain,
Lindley Nic D.
Publication year - 1993
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580331108
Subject(s) - chemistry , pichia pastoris , ergosterol , methanol , deuterium , pichia , yeast , fermentation , biomass (ecology) , chromatography , food science , biochemistry , organic chemistry , recombinant dna , biology , physics , quantum mechanics , agronomy , gene
Three strains of methylotrophic yeasts, Candida boidinii, Pichia angusta (previously Hansenula polymorpha ) and Pichia Pastoris , were studied for their capacity to grow on methanol in deuterated media. Growth rates, determined relative to the extent of deuteration of water and/or methanol, showed that water deuteration was the major limiting factor. After adaptation to deuterium by progressive transfer through media of increasing deuteration, growth rates were diminished relative to those obtained on hydrogenated media of identical salts composition: the two Pichia species retained the highest growth rates (μ=0.02 h −1 ) in the fully deuterated medium. Perdeuterated biomass (16 g) was obtained in a 1 liter fed‐batch fermentation and the extent of deuteraton of isolated ergosterol has been shown to be 97.5% by mass spectrometric analysis.