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133 Ba cryptate labeled immunoglobulin G
Author(s) -
Pettit William A.,
Swailes Barbara K.
Publication year - 1993
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580330409
Subject(s) - chemistry , bioconjugation , cryptand , yield (engineering) , divalent , immunoglobulin g , nuclear chemistry , antibody , combinatorial chemistry , organic chemistry , ion , immunology , metallurgy , materials science , biology
A method for the attachment of cryptates containing radioactive metal ions to proteins has been developed. The aminocryptand, 4,7,13,16,21,24‐hexaoxa‐5,6‐(4′‐aminobenzo)1,10‐diazabicyclo[8.8.8]‐hexacosane, was complexed with divalent 133 Ba and subsequently converted to the isothiocyanatocryptate. The bioconjugate with immunoglobulin G was formed in better than 40% radiochemical yield in a three step procedure. Alternatively, the radiolabeled bioconjugate could be formed by addition of 133 Ba to immunoglobulin G that had been reacted with the isothiocyanate derivative of the cryptand. Stability studies indicated the radiolabeled bioconjugate has a half‐life of 6 h and 12 h in phosphate buffers of pH=7.4 and pH=9.2, respectively. Attempts to label the immunoglobulin with the corresponding 142 Pr cryptate resulted in considerable radioactive colloid formation with no detectable bioconjugate produced.