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Stereoselective synthesis of stable isotope labeled L‐α‐amino acids: The enzymatic preparation of 13 C‐labeled L‐glutamic acids
Author(s) -
Goux Warren J.,
Rench Linda,
Weber Denise S.
Publication year - 1993
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580330304
Subject(s) - chemistry , glutamic acid , enzyme , malate dehydrogenase , amino acid , organic chemistry , biochemistry
We have developed methods for the preparation of L‐glutamic acid, isotopically labeled with 13 C, using commercially available purified enzymes. The procedure is sufficiently versatile that L‐glutamic acid may be labeled at any carbons or any combination of carbons using 13 C‐labeled pyruvate, acetate and bicarbonate as isotopically labeled precursors. Using the strategy outlined, we have demonstrated the methodology using as an example the preparation of millimolar quantities of L‐[1‐ 13 C], L‐[4‐ 13 C], L‐[5‐ 13 C] and L‐[1,3,4‐ 13 C 3 ]glutamic acid in a one‐pot incubation. We also outline methods for the preparation of 13 C‐labeled L‐malate and citrate.