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An investigation into the catalytic deuteration and tritiation of dehydroproline 9 ‐buserelin
Author(s) -
Oehlke J.,
Brankoff T.,
Schmidt M.,
Brudel M.,
Kertscher U.,
Berger H.
Publication year - 1993
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580330302
Subject(s) - chemistry , buserelin , tritium illumination , catalysis , tritium , deuterium , hydrolysis , radiochemistry , nuclear chemistry , chromatography , organic chemistry , biochemistry , physics , receptor , quantum mechanics , nuclear physics , agonist
For optimizing the catalytic tritiation of peptides, the catalytic deuteration of buserelin and DhP 9 ‐buserelin 1 in DMA after reaction of DhP 9 ‐buserelin were comparable in both solvents. The nonspecific incorporation of deuterium found after deuteration of buserelin was clearly lower after reaction in DMA. Tritiation of DhP 9 ‐buserelin in DMA using 85% tritium gas, with conditions optimized by the deuteration experiments, resulted in a specific radioactivity of 1.3 TBq/mmol. 72 and 13 percent of the incorporated radioactivity were found to be associated with proline and histidine, respectively, after acidic hydrolysis.