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Use of HPLC for increasing the molar specific activities of tritium‐labelled nucleosides
Author(s) -
Kudelin B. K.,
Gavrilina L. V.,
Kaminski Yu. L.
Publication year - 1992
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580310912
Subject(s) - chemistry , tritium , fractionation , elution , chromatography , specific activity , molar , ethanol , high performance liquid chromatography , isotope , molar ratio , radiochemistry , tritiated water , organic chemistry , catalysis , enzyme , medicine , physics , dentistry , nuclear physics , quantum mechanics
The possibility of chromatographic separation of different isotopic forms of tritiated nucleosides has been demonstrated. Using a reversed‐phase column in 3–5% solutions of ethanol in water the labelled nucleosides eluted ahead of corresponding unlabelled analogs. Such isotope effects allow simultaneous fine purification of nucleosides and its fractionation according to molar specific activity value.