Premium
Enzymatic synthesis of [methyl‐ 2 H 3 ] creatinine
Author(s) -
Arcelloni C.,
Paroni R.,
Bonini P. A.,
Magni F.,
Kienle M. Galli
Publication year - 1992
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580310703
Subject(s) - chemistry , methylation , creatine , methyltransferase , methionine , methyl group , creatinine , enzyme , stereochemistry , biochemistry , amino acid , organic chemistry , dna , group (periodic table)
2‐Amino‐1,5‐dihydro‐1‐[methyl‐ 2 H 3 ] 4H‐imidazol‐4‐one ([methyl‐ 2 H 3 ] creatinine) was obtained by acidification and heating (120 °C, 120 min) of the [methyl ‐ 2 H 3 ] N‐amidinosarcosine (creatine) synthesised by methylation of guanidoacetate (GA) with S‐adenosyl‐[methyl‐ 2 H 3 ] L‐methionine ([methyl‐ 2 H 3 ] AdoMet) in the presence of rat liver guanidoacetate methyltransferase (GAA‐MT). Isotopic enrichment was 94.7%.