Premium
Stereoselective synthesis of stable isotope‐labeled L‐α‐amino acids: Biosynthesis of 2 H‐, 13 C‐, and 15 N‐labeled L‐serines
Author(s) -
Hanners John,
Gibson Rowena,
Velarde Karen,
Hammer Judy,
Alvarez Mark,
Griego Janet,
Unkefer Clifford J.
Publication year - 1991
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580290708
Subject(s) - methylotroph , serine , chemistry , biosynthesis , glycine , isotopomers , stereospecificity , stereochemistry , amino acid , tritium , glycine cleavage system , biochemistry , organic chemistry , enzyme , catalysis , molecule , physics , nuclear physics
We have developed a stereospecific biosynthesis of 13 C‐ and 15 N‐labeled L‐serine which involves the serine‐type methylotroph Methylobacterium extorquens AM1. In this biosynthesis, C‐3 of serine is derived from methanol while C‐2, C‐1 and the α‐amino group are derived from glycine. By starting with the appropriate labeling precursor, we can produce any of the 2 H, 13 C and/or 15 N isotopomers of L‐serine. Using a 5‐L culture, 40–50 millimoles of L‐serine are produced; L‐serine and glycine are recovered from the growth medium and then separated chromatographically.