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Synthesis of high specific activity tritium‐labelled chloroethylcyclohexylnitrosourea and its application to the study of dna modification
Author(s) -
Siew Ernest L.,
Habraken Yvette,
Ludlum David B.
Publication year - 1991
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580290212
Subject(s) - chemistry , tritium , yield (engineering) , nitrosourea , specific activity , high performance liquid chromatography , dna , ethanolamine , radiochemistry , chromatography , organic chemistry , biochemistry , enzyme , medicine , materials science , physics , surgery , chemotherapy , nuclear physics , metallurgy
Abstract A small‐scale synthesis of high specific activity, N‐(2‐chloro2‐[ 3 H]‐ethyl)‐N′ ‐cyclohexyl‐N‐nitrosourea ([ 3 H]‐CCNU) has been accomplished from tritium‐labelled ethanolamine. The product is pure by TLC and HPLC analysis and has been used successfully to modify DNA. The overall yield on radioactivity including losses in HPLC purification is approximately 4 percent. The availability of this tritium‐labelled compound makes studies of DNA repair and of cellular resistance to N‐(2‐chloroethyl)‐N′ ‐cyclohexyl‐N‐nitrosourea possible.