Premium
14 C‐labelling of hematopoietic immunomodulator ( Z‐100 ) consisting of polysaccharides obtained from cultivation of Mycobacterium tuberculosis
Author(s) -
Sano H.,
Nagae H.,
Suzuki M.,
Tagashira E.,
Fukasaku N.,
Kimishima S.,
Yugi H.,
Baba S.
Publication year - 1991
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580290203
Subject(s) - chemistry , labelling , arabinose , yield (engineering) , sephadex , chromatography , elution , polysaccharide , mycobacterium tuberculosis , radiochemistry , filtration (mathematics) , nuclear chemistry , tuberculosis , biochemistry , xylose , medicine , materials science , pathology , fermentation , metallurgy , enzyme , statistics , mathematics
Mycobacterium tuberculosis was cultivated in sauton meium contaning 31.4 GBq of 14 C‐glycerol as a source of carbon to obtain 14 C‐labelled form of hematopoietic immunomodulator ( Z‐100 ). 14 C‐Z‐100 was produced from the organisms with 0.11‐0.15 % of radiochemical yield. The specific radioactivity of 14 C‐Z‐100 ranged from 3.3 to 7.5 kBq/eq.μ mole D‐arabinose. 14 C‐Z‐100 was analyzed by gel filtration high performance liquid chromatography using a refractive index ( RI ) detector. The elution pattern of the polysaccharides of 14 C‐Z‐100 agreed closely with thay of Z‐100, indicating that 14 C‐Z‐100 was equivalent to Z‐100 in chemical properties In addition, the elution pattern obtained from the radioactivity of 14 C‐Z‐100 was approximated to that obtained by using a RI detector, indicating that Z‐100 was generally labelled with 14 C. Furthermore, 14 C‐Z‐100 was confirmed to have almost the same interleukin‐3 inducing ability and antitumor activity as those of Z‐100.