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Synthesis of 14 C‐labelled methylglyoxal and S‐D‐lactoylglutathione
Author(s) -
Clelland James D.,
Thornalley Paul J.
Publication year - 1990
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580281215
Subject(s) - methylglyoxal , chemistry , acetone , lactoylglutathione lyase , yield (engineering) , chromatography , stereochemistry , organic chemistry , enzyme , materials science , metallurgy
Abstract [2‐ 14 C]Methylglyoxal and S‐D‐[2‐ 14 C]lactoylglutathione were synthesised from [2‐ 14 C]acetone. Acetone was oxidised to methylglyoxal by selenium dioxide and was purified by distillation (yield: 23% based on acetone). Methylglyoxal was converted to S‐D‐lactoylglutathione by reaction with reduced glutathione catalysed by glyoxalase I (EC 4.4.1.5). S‐D‐Lactoylglutathione was purified by anion exchange chromatography on Dowex 1 (yield: 82%, based on methylglyoxal).