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[ 14 C] acetylation of a glycosaminoglycan sulphate: Sulodexide
Author(s) -
Breccia A.,
Gattavecchia E.,
Fini A.,
Marchi E.,
Tamag G. F.
Publication year - 1990
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580280510
Subject(s) - chemistry , heparin , glycosaminoglycan , acetic anhydride , acetylation , dermatan sulfate , fraction (chemistry) , biochemistry , chromatography , heparan sulfate , gene , catalysis
The synthesis of [ 14 C] labelled Sulodexide is reported. Sulodexide is a sulphated polysaccharide of the class of glycosaminoglycan, containing a heparin like fraction (70%), dermatan sulphate (20%) and other minor fractions. The heparin‐like fraction, suitably isolated from other components, was partially and selectively N‐desulphated, thus making few percent unit ‐NH 2 groups available for the labelling with [ 14 C]‐acetic anhydride (specific activity 0.15 μCi/mg). Due to the small extent of modification, the sulphate to carboxylate group ratio remained practically unchanged on the heparin‐like fraction. Sulodexide was reconstituted adding to the labelled fraction the suitable amount of the other components; the chemical and biological properties of the final labelled Sulodexide were undistinguishable from those of the starting material.