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Enzymatic synthesis of [ 11 C] N ‐acetylserotonin
Author(s) -
Mannens Geert,
Slegers Guido,
Goethals Patrick
Publication year - 1990
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580280308
Subject(s) - chemistry , enzyme , bromide , immobilized enzyme , specific activity , nuclear chemistry , radiochemistry , chromatography , medicinal chemistry , stereochemistry , organic chemistry
An enzymatic synthesis of [ 11 C] N ‐acetylserotonin is described. [1‐ 11 C] acetate is produced by reacting 11 CO 2 with methylmagnesium bromide and converted to [ 11 C]acetylCoA by passage over a first enzyme reactor containing immobilized acetylcoenzyme A synthetase. The produced [ 11 C]‐acetylCoA is converted to [ 11 C] N ‐acetylserotonin with a second enzyme reactor containing immobilized acetylcoenzyme A: arylamine N ‐acetyltransferase. The labelled end product is purified by means of ionexchange chromatography and is obtained in a solution suitable for intravenous injection. After irradiation with 18 MeV protons at 15 μA for 20 min, 25 mCi with a specific activity of 250 mCi/μmol were obtained. The whole synthesis starting from EOB takes 40 min.