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Synthesis of high specific active tritiated Leu‐enkephalin in the leucine residue
Author(s) -
Baba S.,
Hasegawa H.,
Shinohara Y.
Publication year - 1989
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580271203
Subject(s) - chemistry , tritium , leu enkephalin , residue (chemistry) , hydrolysis , peptide , enkephalin , leucine , chromatography , amino acid , peptide synthesis , radiochemistry , organic chemistry , biochemistry , receptor , physics , opioid , nuclear physics
Abstract Leu‐enkephalin labelled with tritium in the Leu residue has been prepared. Synthesis of the precursor peptide, [4,5‐dehydro Leu 5 ‐]Leu‐enkephalin, was carried out by solid phase synthesis using Fmoc amino acid derivatives. The peptide was tritiated catalytically yielding 3 H‐Leu‐enkephalin with a specific radioactivity of 4.39 TBq/mmol. The distribution of tritium label was investigated by reversed‐phase high performance liquid chromatography with a synchronized accumulating radioisotope detector following acidic and enzymatic hydrolysis, which confirmed that the tritium label was entirely located at Leu residue.