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Radioiododestannylation. Convenient synthesis of a stable penicillin derivative for rapid penicillin binding protein (PBP) assay
Author(s) -
Blaszczak Larry C.,
Halligan Noreen G.,
Seitz David E.
Publication year - 1989
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580270406
Subject(s) - penicillin , chemistry , penicillin binding proteins , derivative (finance) , antibiotics , biochemistry , chromatography , microbiology and biotechnology , financial economics , economics , biology
Radioiodination of p ‐(trimethylstannyl)penicillin V with [ 125 I]Na using a modification of the chloramine‐T method is simple, high yielding, and site‐specific. The structure and penicillin binding protein (PBP) affinity of p ‐[ 125 l]‐penicillin V (IPV) are similar to penicillin G and the product can be used directly without purification in the PBP assay. Because of the high degree of stability toward autoradiolysis and equivalent PBP binding affinity, IPV can be used in place of [ 3 H]‐penicillin G or [ 14 C]‐penicillin G for these experiments.