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Immunoaffinity methods in radiolabelling of antibodies. A critical assessment using i‐125‐anti‐β‐chorionic gonadotropin as an example
Author(s) -
LiCheng Fu,
Andres Roger Y.
Publication year - 1988
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580250909
Subject(s) - chemistry , labelling , biological activity , antibody , affinity chromatography , antigen , human chorionic gonadotropin , specific activity , elution , chromatography , biochemistry , enzyme , hormone , in vitro , immunology , biology
The usefulness of immunoaffinity methods for the preparation of biologically fully active radiolabelled antibodies was investigated, using anti‐β‐chorionic gonadotropin as a model substance. Immunoaffinity purification increased the fraction of antigen binding antibody after previous labelling from 25 % to 81 %. Radiolabelling of anti‐β‐chorionic gonadotropin while bound to the antigen resulted in biologically highly active material, possibly due to the protection of the immunologically active sites on the antibody during the labelling procedure. Immunoaffinity purification prior to labelling did not result in biologically active material. Elution yields from immunoaffinity supports were generally low.