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Enzymatic synthesis of carbon‐11 acetyl coenzyme A
Author(s) -
Mannens Geert,
Slegers Guido,
Lambrecht Raf,
Goethals Patrick
Publication year - 1988
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580250702
Subject(s) - chemistry , cofactor , acetic acid , coenzyme a , methanol , reagent , bromide , organic chemistry , enzyme , hydrolysis , acetyl coa , chromatography , reductase
An enzymatic synthesis of [ 11 C]acetyl coenzyme A from [1‐ 11 C]acetate is described. 11 CO 2 was reacted with methylmagnesium bromide in diethyl ether at 0°C. The excess of Grignard reagent was hydrolyzed by adding methanol. After evaporation of the solvents, the residue was redissolved in hydrochloric acid and [1‐ 11 C]acetic acid was removed by acid distillation. In the presence of cofactors the [1‐ 11 C] acetic acid was sent over an enzyme reactor containing immobilized acetyl coenzyme A synthetase (Acetate : CoA ligase (AMP forming) EC 6.2.1.1).