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Kit type labelling of [ 123 I] metaiodobenzylguanidine
Author(s) -
Dougan H.,
Lyster D. M.,
Vincent J. S.,
Rihela T.
Publication year - 1988
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580250507
Subject(s) - chemistry , labelling , reagent , radiochemistry , yield (engineering) , aqueous solution , solvent , specific activity , nuclear chemistry , chromatography , organic chemistry , biochemistry , enzyme , materials science , metallurgy
We have examined the labelling of [ 123 I] MIBC by a Cu(I) catalyzed reaction in aqueous solution. We regularly obtained >99% radiochemical purity [ 123 I] MIBC product and >97% radiochemical yield. At 100°C with 2 mg MIBG the radiochemical purity reached 85% at 2.5 min, 97% at 5 min and 99% at 10 min through 30 min. With 200 μg MIBG the reaction proceeded at roughly one tenth the former speed. Many details of technique were essential; the highlights were use of dry triturated reagents and the use of H 2 SO 4 solvent. Neglect of proper procedure led to low radiochemical yields and 123 I bound to apparatus. The procedure has been utilized with up to 1.0 mL 123 I (0.1N NaOH) or 93 mCi 123 I. A study of temperature dependence showed that the reaction proceeded readily, albeit more slowly at 20°C. The temperature coefficient at 100°C was estimated ∼1.7.