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15 N ‐ tomatine
Author(s) -
Elliger Carl A.
Publication year - 1988
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580250307
Subject(s) - chemistry , yield (engineering) , mass spectrometry , sephadex , chromatography , organic chemistry , materials science , metallurgy , enzyme
A method for preparative isolation of 15 N‐tomatine from foliage of tomato plants grown hydroponically with 15 N‐containing nutrient salts is described. Extractive workup of plant material gave a crude product which was chromatographed on Sephadex LH‐20 to yield pure tomatine. Assay of 15 N content by mass spectrometry showed that isotopic purity was ca . 95%.