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Preparation of tritium‐labeled diosgenin, esmilagenin and tigogenin
Author(s) -
Rabinowitz Joseph L.,
Zagoya Juan C. Diaz
Publication year - 1987
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580241104
Subject(s) - diosgenin , chemistry , sapogenin , silica gel , chromatography , gas chromatography , column chromatography , tritium illumination , tritium , ethyl acetate , organic chemistry , medicine , physics , alternative medicine , pathology , nuclear physics
The synthesis of 3 H ‐diosgenin, 3 H ‐esmilagenin and 3 H ‐tigogenin (three potential hypocholesterolemic agents) was accomplished by low pressure hydrogenation of commercial (Sigma D 1634) diosgenin with 3 H ‐gas. The mixture of 3 H materials obtained was separated by silica gel G‐60 column chromatography by use of a benzene: ethyl acetate (8:2 v/v) solution. Purification of each sapogenin was by TLC chromatography.