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Optimum labelling of monoclonal antibody 3El.2 with 111 in using a bifunctional chelate
Author(s) -
Reilly R. R.,
Ege G. G.
Publication year - 1987
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580240510
Subject(s) - chemistry , labelling , bifunctional , sephadex , chelation , chromatography , monoclonal antibody , conjugate , molar ratio , nuclear chemistry , radiochemistry , antibody , organic chemistry , biochemistry , enzyme , catalysis , mathematical analysis , mathematics , immunology , biology
Monoclonal antibody 3El.2 was coupled with the bifunctional chelate, cyclic DTPA anhydride, at molar ratios (cDTPAA:3El.2) of 1:1, 10:1, 100:1 and 1000:1. The desired substitution level of less than one mol of DTPA/mol of 3El.2 was achieved at a molar ratio of 10:1 and a coupling efficiency of 9.9±3.38 percent. The DTPA‐coupled antibody was purified by dialysis before labelling with 111 In acetate. The labelling efficiency was 38.7±3.59 percent. The radiolabelled antibody was purified by PD‐10 Sephadex G‐25M chromatography then sterilized by filteration through a 0.22 um filter (Milex‐GS or Millex‐GV). A substantial adsorption effect (87.4±3.84 percent) occurs with the Millex‐GS filter but to a much smaller extent (26.8±5.58 percent) with the Millex‐GV filter. The radiochemical purity of the product was 80–90 percent depending on the method of analysis. The product was tested and found to be sterile and non‐pyrogenic.