Premium
A simple procedure for the preparation of tritiated homovanillic acid
Author(s) -
Keenan Roy W.
Publication year - 1987
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580240311
Subject(s) - chemistry , homovanillic acid , tritium , tritiated water , yield (engineering) , deuterium , sulfuric acid , radiochemistry , tritium illumination , specific activity , heavy water , organic chemistry , acetic acid , chromatography , biochemistry , enzyme , physics , receptor , materials science , quantum mechanics , nuclear physics , serotonin , metallurgy
Homovanillic acid (4‐hydroxy‐3‐methoxyphenylacetic acid) was labeled with tritium by exchange reaction in tritiated water catalyzed by sulfuric acid. The product was obtained in good yield and specific activities sufficient be utilized in metabolic experiments. Reactions carried out under identical conditions, but in the presence of deuterated water gave a product in which all the aromatic hydrogens and those α ‐ to the carboxyl group were replaced with deuterium indicating that the tritium‐labeled homovanillic acid was labeled in these positions.