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Synthesis of diethylene triamine pentaacetic acid (DTPA) conjugated antibodies
Author(s) -
Keeling A. A.,
Bradwell A. A.
Publication year - 1987
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580240111
Subject(s) - chemistry , conjugate , chelation , conjugated system , in vivo , antibody , in vitro , combinatorial chemistry , size exclusion chromatography , chromatography , organic chemistry , biochemistry , polymer , mathematical analysis , biology , enzyme , mathematics , microbiology and biotechnology , immunology
Antibodies labelled with radioisotopes via chelating agents are being used increasingly for in vivo localisation or radiotherapy of tumours. The most widely used chelating agent is DTPA. Here, we describe technical modifications of the standard DTPA cyclic anhydride conjugation procedure and determine the in vitro stability of the DTPA‐antibody conjugates. The use of this derivative of DTPA combined with the use of appropriate buffers and small disposable gel filtration columns resulted in the development of a simpler and more rapid technique for the production of DTPA‐conjugated antibodies.