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Ochratoxin A labelled with 14 C or 3 H in the phenylalanine moiety
Author(s) -
Hult Karl
Publication year - 1986
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580230802
Subject(s) - ochratoxin a , chemistry , phenylalanine , ochratoxin , mycotoxin , moiety , hydrolysis , isocoumarins , organic chemistry , biochemistry , amino acid , food science , catalysis
Ochratoxin A is a mycotoxin produced by Aspergillus and Penicillium moulds. The toxin is an amide formed between phenylalanine and the isocoumarin acid ochratoxin α. Ochratoxin A with high specific radioactivity can be obtained, by substitution of the natural phenylalanine for 14 C or 3 H‐labelled phenylalanine. Labelled ochratoxin A was formed by condensation of labelled phenylalanine ethylester with an anhydride derivative of ochratoxin α. The intermediate O‐acetyl ochratoxin C was hydrolyzed with pig liver esterase and chymotrypsin to afford ochratoxin A. The overall yield of radioactivity was 50%. Specific activities up to 500 Ci/mol and 100 Ci/mmol for 14 C and 3 H respectively were obtained.