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Radiolysis of D(+)‐carnitine by 60 Co‐γ‐radiation and formation of L(+)‐β‐methylcholine
Author(s) -
Löster Heinz,
Strack Erich,
Seim Hermann
Publication year - 1986
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580230606
Subject(s) - chemistry , radiolysis , carnitine , ion chromatography , radiation chemistry , irradiation , nuclear chemistry , chromatography , organic chemistry , chemical reaction , biochemistry , aqueous solution , nuclear physics , physics
The radiolysis of D(+)‐carnitine by 60 Co‐γ‐radiation was examined to obtain optically active β‐methylcholine. It was found that the radio lysis leads to a number of trimethylammonium bases but to no other betaines. The acids formed were not investigated. The bases produced were separated by ion exchange chromatography. (+)‐β‐Methylcholine and acetonyltrimethylammonium could be identified by means of common analytical methods (IR, NMR, MS and MA) applied to the fractions. The amounts of methylamines formed by irradiation, and determined by gas chromatography, were very small. Racemization of the D(+)‐carnitine did not occur during irradiation, L(‐)‐carnitine was not found when an enzymatical determination method was used. The fact that (+)‐β‐methylcholine was formed from D(+)‐carnitine is pharmacologically important, because acetyl‐L(+)‐β‐methylcholine has a strong interaction with muscarinic receptors.