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Preparation and purification of 7‐Iodoclonazepam for use in Radioimmunoassay
Author(s) -
Goddard C P,
Law B,
Mason P A,
Stead A H
Publication year - 1986
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580230406
Subject(s) - radioimmunoassay , chemistry , chromatography , antiserum , mass spectrometry , biochemistry , antigen , genetics , biology
A method is described for the preparation and purification of 7‐[ 125 I]‐Iodoclonazepam (5‐( o ‐Chlorophenyl)‐2, 3‐Dihydro‐7‐[ 125 I]‐Iodo‐1H‐1,4‐Benzodiazepin‐2‐one). The structure was confirmed by mass spectrometry using 7‐[ 127 I]iodoclonazepam prepared by the same method. 7‐[ 125 I]‐Iodoclonazepam binds well to a benzodiazepine antiserum. Although readily displaced by all the benzodiazepines commercially available in the UK, it is not displaced by structurally related nonbenzodiazepines except at very high concentrations. 7‐[ 125 I]Iodoclonazepam should therefore be useful for the development of a screening radioimmunoassay (RIA) for benzodiazepines.