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[ 11 C]Labeling of coenzyme Q 10 and its tissue distribution
Author(s) -
Takahashi T.,
Ido T.,
Iwata R.,
Ishiwata K.,
Hamamura K.,
Kogure K.
Publication year - 1985
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580220606
Subject(s) - chemistry , coenzyme a , cofactor , lipophilicity , coenzyme q – cytochrome c reductase , distribution (mathematics) , stereochemistry , radiochemistry , biochemistry , enzyme , mitochondrion , mathematical analysis , cytochrome c , mathematics , reductase
[ 11 C]Coenzyme Q 10 was synthesized by O‐methylation of 3‐demethyl coenzyme Q 10 with 11 CH 3 I. We have produced 1–4 mCi of [ 11 C]coenzyme Q 10 with radiochemical yields of 6–16 % (based on trapping 11 CH 3 I) in 35–50 min with radiochemical purities of >95%. The specific activity was 4–5 mCi/μMol at the end of [ 11 C]‐coenzyme Q 10 synthesis. The tissue distribution was investigated on mongolian gerbils after intravenous administration of [ 11 C]‐coenzyme Q 10 solubilized with polyoxyethylene hydrogenated caster oil (HCO‐60). The blood level of [ 11 C]coenzyme Q 10 was very high and its clearance was slow. The accumulations in the heart, kidney and liver were moderate and the accumulation in the brain was low in spite of its high lipophilicity.