Premium
The preparation of carbon‐14 labeled avermectin B 1 a
Author(s) -
Ku C. C.,
Hwang S. C.,
Kaplan L.,
Nallin M. K.,
Jacob T. A.
Publication year - 1985
Publication title -
journal of labelled compounds and radiopharmaceuticals
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.432
H-Index - 47
eISSN - 1099-1344
pISSN - 0362-4803
DOI - 10.1002/jlcr.2580220506
Subject(s) - chemistry , avermectin , propionate , high performance liquid chromatography , carbon 14 , chromatography , carbon fibers , fermentation , organic chemistry , medicine , physics , materials science , quantum mechanics , composite number , composite material , anatomy
Carbon‐14 labeled avermectin B 1 a was synthesized by a fermentation process using sodium [1‐ 14 C]propionate as the precursor, then isolated and purified by preparative HPLC. The total amount of purified product obtained in this preparation was 225 mg with a specific activity of 16.4 μCi/mg. The radiopurity was 99 + % by TLC and HPLC analyses. The labeled positions were determined by carbon‐13 NMR analysis of [ 13 C]propionate. The labeled atoms were determined to be C‐3, C‐7, C‐11, C‐13 and C‐23. However, EI/MS indicated this product was a mixture of five single carbon‐13 labeled compounds. The overall radiochemical yeild of [ 14 C]avermectin B 1 a biosynthesis was estimated to be 0.69%.